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Is it possible for a region to work or stop working after a certain period of time? Like a damage region for instance. Thanks in advance.

There's a "RemoveRegion" .lua callback. You can always set up a timer in the .lua and on the end of the timer trigger "RemoveRegion." The only caveat is that I'm not sure if/how exactly RemoveRegion works.

Have you tired disabling it after the timer ends?

I haven't tried using it at all, timer or no timer. I would assume that it would work on a timer end if it would work at all.